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cfx96 touchtm real time fluorescent quantitative pcr instrument  (Bio-Rad)


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    Bio-Rad cfx96 touchtm real time fluorescent quantitative pcr instrument
    Cfx96 Touchtm Real Time Fluorescent Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+touchtm+real+time+pcr+detection+system+instrument/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41900994-158-6-13
    Average 96 stars, based on 27901 article reviews
    cfx96 touchtm real time fluorescent quantitative pcr instrument - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: PPAR Receptors Expressed from Vectors Containing CMV Promoter Can Enhance Self-Transcription in the Presence of Fatty Acids from CLA-Enriched Egg Yolks-A Novel Method for Studies of PPAR Ligands.
    Article Snippet: PPAR receptors are ligand-dependent transcription factors activated in response to various small lipophilic ligands controlling the expression of different genes involved in cellular differentiation, development, metabolism, and tumorigenesis.. Unexpectedly, our previous studies have shown that single plasmid-based expression of PPARs under the control of CMV promoter/enhancer was significantly elevated in the presence of PPAR agonists.. Here we show that the PPAR reporters controlled by the CMV promoter/enhancer, that was shown to contain three internal non-canonical PPRE elements, can be used as a fast screening system for more effective PPAR ligands.

    Article Title: Young Shoots of Red Beet and the Root at Full Maturity Inhibit Proliferation and Induce Apoptosis in Breast Cancer Cell Lines.
    Article Snippet: The reverse transcriptase (RT) reaction was performed to synthesize cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. Quantitative verification of gene expression was performed using the CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, Hercules, CA, USA). .. The PCR reaction mixture contained cDNA samples, RNase-free water, SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), and primers for the following genes: apoptosisinducing factor mitochondria associated 1 (AIFM1), AKT serine/threonine kinase 1 (AKT1), apoptotic peptidase activating factor 1 (Apaf-1), Bcl2-associated agonist of cell death (BAD), Bcl2- binding component 3 (BBC3), B Cell Lymphoma 2 (BCL2), H3-interacting domain death agonist (BID), caspase-3 (CASP-3), caspase-7 (CASP-7), caspase-8 (CASP-8), diablo IAP-binding mitochondrial protein (DIABLO), Fas-associated death domain (FADD), Fas cell surface death receptor (FAS), v-myc myelocytomatosis viral oncogene homolog (MYC), nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), PH domain and leucine-rich repeat protein phosphatase 1 (PHLPP1) and tumor protein p53 (TP53).

    Article Title: Dietary conjugated linoleic acid affects blood parameters, liver morphology and expression of selected hepatic genes in laying hens.
    Article Snippet: The objective of this research were to investigate the effect of a conjugated linoleic acid (CLA)-enriched diet on Isa Brown laying hen health status and to provide a comprehensive analysis of changes in blood parameters, liver morphology and selected hepatic gene expression.. Hens were allocated to the control and experimental group (diet enriched with 0.75% CLA) for a total period of 4 months.. At the end of the experiment half of the hens from each group were slaughtered for analyses.

    Article Title: Methane fluxes from coastal sediments are enhanced by macrofauna
    Article Snippet: The standard curve was calibrated in ten-fold dilutions ranging from 10 0 to 10 −5 using DNA from Methanoculleus marisnigrii (DSMZ 1498). .. All reactions were realized in 96 well Q-PCR plates using CFX96 TouchTM Real-Time PCR Detection System Instrument (C1000 TouchTM Thermal, Cycler, Bio-Rad) and its software. ..

    Article Title: Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells.
    Article Snippet: To synthesize cDNA, the reverse transcriptase (RT) reaction was performed using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. A quantitative analysis of gene expression was carried out in CFX96 TouchTM Real-Time PCR Detection System instrument on prime PCR custom plates with the use of SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Neutrophils contribute to the pathogenesis of hemorrhagic cystitis induced by ifosfamide.
    Article Snippet: Ifosfamide (IFO) is an antineoplastic drug that is commonly used to treat gynecological and breast cancers.. Hemorrhagic cystitis (HC) is a common side effect associated with IFO injection, which courses with neutrophil accumulation and affects 6–50% of patients depending on dose intensity.. Here, we investigated the role of neutrophils in this inflammatory process.

    Article Title: Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
    Article Snippet: Reverse transcription was performed on 1 μ g of total RNA using Maxima First-Strand cDNA Synthesis kit for RT-qPCR (Thermo Scientific, MA, USA). .. Quantitative verification of genes was performed using CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, CA, USA) and SYBR Green Precision Melt Supermix kit (Bio-Rad, CA, USA). ..

    Article Title: Young Shoots of Red Beet and the Root at Full Maturity Inhibit Proliferation and Induce Apoptosis in Breast Cancer Cell Lines
    Article Snippet: The reverse transcriptase (RT) reaction was performed to synthesize cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. Quantitative verification of gene expression was performed using the CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, Hercules, CA, USA). .. The PCR reaction mixture contained cDNA samples, RNase-free water, SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), and primers for the following genes: apoptosis-inducing factor mitochondria associated 1 (AIFM1), AKT serine/threonine kinase 1 (AKT1), apoptotic peptidase activating factor 1 (Apaf-1), Bcl2-associated agonist of cell death (BAD), Bcl2- binding component 3 (BBC3), B Cell Lymphoma 2 (BCL2), H3-interacting domain death agonist (BID), caspase-3 (CASP-3), caspase-7 (CASP-7), caspase-8 (CASP-8), diablo IAP-binding mitochondrial protein (DIABLO), Fas-associated death domain (FADD), Fas cell surface death receptor (FAS), v-myc myelocytomatosis viral oncogene homolog (MYC), nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), PH domain and leucine-rich repeat protein phosphatase 1 (PHLPP1) and tumor protein p53 (TP53).

    SYBR Green Assay:

    Article Title: PPAR Receptors Expressed from Vectors Containing CMV Promoter Can Enhance Self-Transcription in the Presence of Fatty Acids from CLA-Enriched Egg Yolks-A Novel Method for Studies of PPAR Ligands.
    Article Snippet: PPAR receptors are ligand-dependent transcription factors activated in response to various small lipophilic ligands controlling the expression of different genes involved in cellular differentiation, development, metabolism, and tumorigenesis.. Unexpectedly, our previous studies have shown that single plasmid-based expression of PPARs under the control of CMV promoter/enhancer was significantly elevated in the presence of PPAR agonists.. Here we show that the PPAR reporters controlled by the CMV promoter/enhancer, that was shown to contain three internal non-canonical PPRE elements, can be used as a fast screening system for more effective PPAR ligands.

    Article Title: Dietary conjugated linoleic acid affects blood parameters, liver morphology and expression of selected hepatic genes in laying hens.
    Article Snippet: The objective of this research were to investigate the effect of a conjugated linoleic acid (CLA)-enriched diet on Isa Brown laying hen health status and to provide a comprehensive analysis of changes in blood parameters, liver morphology and selected hepatic gene expression.. Hens were allocated to the control and experimental group (diet enriched with 0.75% CLA) for a total period of 4 months.. At the end of the experiment half of the hens from each group were slaughtered for analyses.

    Article Title: Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells.
    Article Snippet: To synthesize cDNA, the reverse transcriptase (RT) reaction was performed using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. A quantitative analysis of gene expression was carried out in CFX96 TouchTM Real-Time PCR Detection System instrument on prime PCR custom plates with the use of SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Neutrophils contribute to the pathogenesis of hemorrhagic cystitis induced by ifosfamide.
    Article Snippet: Ifosfamide (IFO) is an antineoplastic drug that is commonly used to treat gynecological and breast cancers.. Hemorrhagic cystitis (HC) is a common side effect associated with IFO injection, which courses with neutrophil accumulation and affects 6–50% of patients depending on dose intensity.. Here, we investigated the role of neutrophils in this inflammatory process.

    Article Title: Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
    Article Snippet: Reverse transcription was performed on 1 μ g of total RNA using Maxima First-Strand cDNA Synthesis kit for RT-qPCR (Thermo Scientific, MA, USA). .. Quantitative verification of genes was performed using CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, CA, USA) and SYBR Green Precision Melt Supermix kit (Bio-Rad, CA, USA). ..

    Gene Expression:

    Article Title: Young Shoots of Red Beet and the Root at Full Maturity Inhibit Proliferation and Induce Apoptosis in Breast Cancer Cell Lines.
    Article Snippet: The reverse transcriptase (RT) reaction was performed to synthesize cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. Quantitative verification of gene expression was performed using the CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, Hercules, CA, USA). .. The PCR reaction mixture contained cDNA samples, RNase-free water, SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), and primers for the following genes: apoptosisinducing factor mitochondria associated 1 (AIFM1), AKT serine/threonine kinase 1 (AKT1), apoptotic peptidase activating factor 1 (Apaf-1), Bcl2-associated agonist of cell death (BAD), Bcl2- binding component 3 (BBC3), B Cell Lymphoma 2 (BCL2), H3-interacting domain death agonist (BID), caspase-3 (CASP-3), caspase-7 (CASP-7), caspase-8 (CASP-8), diablo IAP-binding mitochondrial protein (DIABLO), Fas-associated death domain (FADD), Fas cell surface death receptor (FAS), v-myc myelocytomatosis viral oncogene homolog (MYC), nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), PH domain and leucine-rich repeat protein phosphatase 1 (PHLPP1) and tumor protein p53 (TP53).

    Article Title: Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells.
    Article Snippet: To synthesize cDNA, the reverse transcriptase (RT) reaction was performed using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. A quantitative analysis of gene expression was carried out in CFX96 TouchTM Real-Time PCR Detection System instrument on prime PCR custom plates with the use of SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Young Shoots of Red Beet and the Root at Full Maturity Inhibit Proliferation and Induce Apoptosis in Breast Cancer Cell Lines
    Article Snippet: The reverse transcriptase (RT) reaction was performed to synthesize cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. Quantitative verification of gene expression was performed using the CFX96 TouchTM Real-Time PCR Detection System instrument (Bio-Rad, Hercules, CA, USA). .. The PCR reaction mixture contained cDNA samples, RNase-free water, SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), and primers for the following genes: apoptosis-inducing factor mitochondria associated 1 (AIFM1), AKT serine/threonine kinase 1 (AKT1), apoptotic peptidase activating factor 1 (Apaf-1), Bcl2-associated agonist of cell death (BAD), Bcl2- binding component 3 (BBC3), B Cell Lymphoma 2 (BCL2), H3-interacting domain death agonist (BID), caspase-3 (CASP-3), caspase-7 (CASP-7), caspase-8 (CASP-8), diablo IAP-binding mitochondrial protein (DIABLO), Fas-associated death domain (FADD), Fas cell surface death receptor (FAS), v-myc myelocytomatosis viral oncogene homolog (MYC), nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), PH domain and leucine-rich repeat protein phosphatase 1 (PHLPP1) and tumor protein p53 (TP53).

    Software:

    Article Title: Methane fluxes from coastal sediments are enhanced by macrofauna
    Article Snippet: The standard curve was calibrated in ten-fold dilutions ranging from 10 0 to 10 −5 using DNA from Methanoculleus marisnigrii (DSMZ 1498). .. All reactions were realized in 96 well Q-PCR plates using CFX96 TouchTM Real-Time PCR Detection System Instrument (C1000 TouchTM Thermal, Cycler, Bio-Rad) and its software. ..

    Polymerase Chain Reaction:

    Article Title: Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells.
    Article Snippet: To synthesize cDNA, the reverse transcriptase (RT) reaction was performed using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). .. A quantitative analysis of gene expression was carried out in CFX96 TouchTM Real-Time PCR Detection System instrument on prime PCR custom plates with the use of SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). ..



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